Head of Laboratory:
Assoc. Prof. Mongkol Kunakorn, M.D.,
Certificate of Proficiency in Clinical Pathology
Rheumatoid arthritis (RA) is a systemic immune disorder which mainly results in joint inflammation. Disease-modifying antirheumatic drugs (DMARDs) are a group of medication for treating RA. RA patients with DMARDs reduction needs a tool for careful monitoring. Our project aimed to determine the number and suppressive activity of Treg and plasma cytokines from peripheral blood (PB) of RA patients with DMARDs reduction.
Immune deficiency & Allergy
Severe combined immunodeficiency (SCID) is a heterogeneous group of primary immune deficiency disorders. We developed a real time PCR assay to detect T cell receptor excision circles (TREC). TREC are small circular fragments of DNA that form as T cell receptor rearrangement occurs during maturation of T cells in the thymus. Once a child is identified to have very low to absent TREC, further diagnostic tests including flow cytometry to quantify total T cells (CD3), helper T cells (CD4), suppressor T cells (CD8), B cells (CD19/20), naïve T cells (CD45 RA), memory T cells (CD45 RO) and NK cells (CD 16/56) is performed.
Changes of Regulatory T cells after Allergen-specific Subcutaneous Immunotherapy, Rush Protocol, in Children. Moreover, we interest immunological biomarker for diagnosis of Hypersensitivity pneumonitis (HP) in patient with a fibrosing alveolitis produced by inhalation of diverse antigens.
Immuno-Oncology Research Using Flow Cytometry
Immuno-oncology is the study of the immune system within a tumor microenvironment. Identification of biomarkers in cancer immunotherapy that predict therapeutic response and/or limit adverse events are a critical need in the field. We focus on renal cell carcinoma and lung cancer.
We recently establish use flow cytometry for quantitative measure immune cells, especially regulatory T cell (Treg), T cell CD8+ PD1+ etc. Moreover, the function of Treg by suppressing assay are evaluated and correlated with clinical characteristics for cancer patients. Including the immune checkpoint inhibitor (anti-PD1, anti-CTLA4) and cytokines panels.
Research application of viral serology including HIV, Hepatitis virus (HAV, HBV, HCV), TORCH infection, Bacterial serology e.g. Melioid titer, ASO titer, Leptospira Ab, Scrub typhus Ab, C. difficile toxin, H. pyroli (Ab/Ag), fungal serology e.g. cryptococcus Ag, galactomannan, mannan (candida Ag), and Pneumocystis jiroveci (by IF), serologic testing for syphilis e.g. VDRL, RPR, TPPA, FTA-ABS, and Syphilis TP (CLIA), Interferon-Gamma Release Assays (IGRAs) included T-SPOT.TB (Tuberculosis), QuantiFERON-TB, and QuantiFERON-CMV.
Microbial pathogenesis & Host responses to endemic pathogens
Infectious diseases pose threats to human health, and this problem has become more concerned, as drug-resistant and endemic pathogens increasingly exist.
Our research focuses on microbial pathogenesis and host responses to endemic pathogens, such as Pythium insidiosum, with attempts to better understand how the disease occurs at the cellular and molecular level.
We also search for a new drug and vaccine target for a particular pathogen. Many sophisticated immunological, biochemical, and molecular techniques have been employed to achieve our research goal.
The obtained knowledge will pave a way to development of effective diagnostic and therapeutic modalities, which could promote a better prognosis of patients with the infectious disease.
So far, we have published a number of publications, regarding about microbial pathogenesis and host responses, in many impacted journals as to demonstrate our contribution to the schematic community.
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